QBEmax is a sequence-permuted and internally protected base editor
Affiliations
- 1 Qi Biodesign, Beijing, China.
- 2 Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
- 3 Qi Biodesign, Beijing, China. [email protected].
- PMID: 40258958
- PMCID: PMC12995723
- DOI: 10.1038/s41587-025-02641-9
Abstract
Cytosine base editors (CBEs) show promise for multiplex gene knockout applications, but impure edits, indels and off-targets still frequently occur. We describe here QBEmax, which exhibits high efficiency, low indel and off-targets and high product purity with up to 99.8% of edits comprised of C-to-T. Through molecular dynamic modeling, QBEmax presents as a compact and stable base editor that shields protected bases from undesired repair processes.