Hsa_circ_0063526 promotes the development of endometriosis by sponging miRNA-141-5p

Background:: Endometriosis can cause various social burdens. Abnormal circular RNAs level lead to changes of related gene expression, thereby mediating the occurrence and development of a series of diseases. The research of circRNA in endometriosis is still in its infancy. This study will explore the role of hsa_circ_0063526 with microRNA-141-5p in the development of endometriosis.

Methods:: The expression of genes was detected by RT-qPCR. Transwell, wound-healing, EdU assay were performed on endometriosis patient’s End1 / E6E7 cell. PCR and immunohistochemistry were used to detect the expression of candidate regulatory genes in ectopic lesions in endometriosis mice model.

Results:: The expression level of hsa_circ_0063526 in endometriosis patients’ ectopic tissue is higher than control(P <0.05), The expression levels of hsa_circ_0063526 and miRNA-141-5P in ectopic tissue of endometriosis were negatively correlated (P < 0.05).Knockdown hsa_circ_0063526 inhibited the invasion, metastasis, and proliferation ability of End1 / E6E7 cell. The inhibition of microRNA-141-5p can rescue this inhibitory effect (P <0.05). In-vivo experiments showed miR-141-5p and si-hsa_circ_0063526 treatment reduced the lesion size and regulated endometriosis genes.

Conclusion:: Hsa_circ_0063526 is probably involved in regulating the occurrence and development of endometriosis by sponging miR-141-5p. hsa_circ_0063526 and miR-141-5p are possible to become biomarkers and therapeutic targets for endometriosis.